NAT8 Mouse Monoclonal Antibody [Clone ID: OTI5A8]

CAT#: CF500544

Carrier-free (BSA/glycerol-free) NAT8 mouse monoclonal antibody, clone OTI5A8 (formerly 5A8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NAT8 (RC203157, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NAT8. Positive lysates LY401303 (100ug) and LC401303 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-NAT8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500544, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-NAT8mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500544, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-NAT8mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500544, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-NAT8mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500544, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-NAT8mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500544, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-NAT8mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500544, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-NAT8mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500544, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-NAT8mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500544, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-NAT8mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500544, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-NAT8mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500544, Dilution 1:50)

• Anti-NAT8 mouse monoclonal antibody (TA500544) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NAT8 (RC203157).

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Specifications

Product Data

• Clone Name: OTI5A8
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:50, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human NAT8 expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25.6 kDa
• Gene Name: Homo sapiens N-acetyltransferase 8 (putative) (NAT8), mRNA.
• Database Link:
  NP_003951
  Entrez Gene 9027 Human
  UniProt ID Q9UHE5
• Background: This gene, isolated using the differential display method to detect tissue-specific genes, is specifically expressed in kidney and liver. The encoded protein shows amino acid sequence similarity to N-acetyltransferases. A similar protein in Xenopus affects cell adhesion and gastrulation movements, and may be localized in the secretory pathway. A highly similar paralog is found in a cluster with this gene.
• Synonyms: ATase2; CCNAT; CML1; GLA; Hcml1; TSC501; TSC510

Reference Data

• Protein Families: Transmembrane