Protein kinase Y linked (PRKY) Mouse Monoclonal Antibody [Clone ID: OTI5E9]

CAT#: CF500559

Carrier-free (BSA/glycerol-free) PRKY mouse monoclonal antibody, clone OTI5E9 (formerly 5E9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKY (RC210438, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKY. Positive lysates LY400977 (100ug) and LC400977 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-PRKYmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500559, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-PRKYmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500559, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-PRKYmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500559, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded lymphoma tissue using anti-PRKYmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500559, Dilution 1:50)

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  Anti-PRKY mouse monoclonal antibody (TA500559) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKY (RC210438).

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Specifications

Product Data

• Clone Name: OTI5E9
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:1000 IHC 1:50 IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human PRKY expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 31.7 kDa
• Gene Name: Homo sapiens protein kinase, Y-linked (PRKY)
• Database Link:
  NP_002751
  Entrez Gene 5616 Human
• Background: This gene is similar to the protein kinase, X-linked gene in the pseudoautosomal region of the X chromsoome. The gene is classified as a transcribed pseudogene because it has lost a coding exon that results in all transcripts being candidates for nonsense-mediated decay (NMD) and unlikely to express a protein.Abnormal recombination between this gene and a related gene on chromosome X is a frequent cause of XX males and XY females.
• Synonyms: OTTHUMP00000033227; protein kinase; Y-linked

Reference Data

• Protein Families: Druggable Genome, Protein Kinase