Cytochrome P450 Reductase (POR) Mouse Monoclonal Antibody [Clone ID: OTI3H10]

CAT#: CF500572

Carrier-free (BSA/glycerol-free) POR mouse monoclonal antibody, clone OTI3H10 (formerly 3H10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POR (RC202172, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POR. Positive lysates LY424436 (100ug) and LC424436 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-PORmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500572, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-PORmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500572, Dilution 1:50)

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  Anti-POR mouse monoclonal antibody (TA500572) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POR (RC202172).

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  HEK293T cells transfected with either pCMV6-ENTRY POR (RC202172) (Red) or empty vector control plasmid (Blue) were immunostained with anti-POR mouse monoclonal (TA500572, Dilution 1:1,000), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI3H10
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:50, IF 1:100, Flow 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human POR expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 76.7 kDa
• Gene Name: Homo sapiens cytochrome p450 oxidoreductase (POR), transcript variant 2, mRNA.
• Database Link:
  NP_000932
  Entrez Gene 18984 MouseEntrez Gene 29441 RatEntrez Gene 5447 Human
  UniProt ID P16435
• Background: This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome.
• Synonyms: CPR; CYPOR; P450R

Reference Data

• Protein Families: Druggable Genome, P450, Transmembrane