PP5 (PPP5C) Mouse Monoclonal Antibody [Clone ID: OTI2E12]

CAT#: CF500585

Carrier-free (BSA/glycerol-free) PPP5C mouse monoclonal antibody, clone OTI2E12 (formerly 2E12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP5C (RC201650, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP5C. Positive lysates LY416767 (100ug) and LC416767 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPP5C monoclonal antibody.

• 

  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-PPP5C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500585)

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PPP5C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500585)

• 

  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PPP5C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500585)

• 

  Immunofluorescent staining of HT29 cells using anti-PPP5C mouse monoclonal antibody (TA500585).

• HEK293T cells transfected with either RC201650 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PPP5C antibody (TA500585), and then analyzed by flow cytometry.

Show all 7 images

Specifications

Product Data

• Clone Name: OTI2E12
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PPP5C (NP_006238) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 56.9 kDa
• Gene Name: Homo sapiens protein phosphatase 5 catalytic subunit (PPP5C), transcript variant 1, mRNA.
• Database Link:
  NP_006238
  Entrez Gene 65179 RatEntrez Gene 612199 DogEntrez Gene 716214 MonkeyEntrez Gene 5536 Human
  UniProt ID P53041
• Background: May play a role in the regulation of RNA biogenesis and/or mitosis. In vitro, dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1.
• Synonyms: PP5; PPP5; PPT

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: MAPK signaling pathway