KDM4C Mouse Monoclonal Antibody [Clone ID: OTI8A1]

CAT#: CF500588

Carrier-free (BSA/glycerol-free) KDM4C mouse monoclonal antibody, clone OTI8A1 (formerly 8A1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KDM4C (RC211182, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KDM4C. Positive lysates LY402405 (100ug) and LC402405 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-KDM4C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500588, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-KDM4C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500588, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-KDM4C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500588, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-KDM4C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500588, Dilution 1:50)

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Specifications

Product Data

• Clone Name: OTI8A1
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:50
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human KDM4C expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 120.0 kDa
• Gene Name: Homo sapiens lysine demethylase 4C (KDM4C), transcript variant 1, mRNA.
• Database Link:
  NP_055876
  Entrez Gene 23081 Human
  UniProt ID Q9H3R0
• Background: This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein with one JmjC domain, one JmjN domain, two PHD-type zinc fingers, and two Tudor domains. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form. Chromosomal aberrations and increased transcriptional expression of this gene are associated with esophageal squamous cell carcinoma. Alternative splicing results in multiple transcript variants.
• Synonyms: GASC1; JHDM3C; JMJD2C; TDRD14C

Reference Data

• Protein Families: Druggable Genome, Transcription Factors