PP5 (PPP5C) Mouse Monoclonal Antibody [Clone ID: OTI3G1]

CAT#: CF500597

Carrier-free (BSA/glycerol-free) PPP5C mouse monoclonal antibody, clone OTI3G1 (formerly 3G1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP5C (RC201650, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP5C. Positive lysates LY416767 (100ug) and LC416767 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC201650 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PPP5C antibody (TA500597), and then analyzed by flow cytometry.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PPP5C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500597)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PPP5C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500597)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PPP5C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500597)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PPP5C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500597)

• Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-PPP5C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500597)

• Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PPP5C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500597)

• Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PPP5C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500597)

• Anti-PPP5C mouse monoclonal antibody (TA500597) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPP5C (RC201650).

• Immunofluorescent staining of HeLa cells using anti-PPP5C mouse monoclonal antibody (TA500597).

• Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPP5C monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

• Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and PPP5C-Knockout 293T cells (KO, Cat# LC811022) were separated by SDS-PAGE and immunoblotted with anti-PPP5C monoclonal antibody TA500597, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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Specifications

Product Data

• Clone Name: OTI3G1
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:50, IF 1:50~100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PPP5C (NP_006238) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 56.7 kDa
• Gene Name: Homo sapiens protein phosphatase 5 catalytic subunit (PPP5C), transcript variant 1, mRNA.
• Database Link:
  NP_006238
  Entrez Gene 65179 RatEntrez Gene 5536 Human
  UniProt ID P53041
• Background: This gene encodes a serine/threonine phosphatase which is a member of the protein phosphatase catalytic subunit family. Proteins in this family participate in pathways regulated by reversible phosphorylation at serine and threonine residues; many of these pathways are involved in the regulation of cell growth and differentiation. The product of this gene has been shown to participate in signaling pathways in response to hormones or cellular stress, and elevated levels of this protein may be associated with breast cancer development. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
• Synonyms: PP5; PPP5; PPT

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: MAPK signaling pathway