PPP1R7 Mouse Monoclonal Antibody [Clone ID: OTI4F9]

CAT#: CF500614

Carrier-free (BSA/glycerol-free) PPP1R7 mouse monoclonal antibody, clone OTI4F9 (formerly 4F9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP1R7 (RC201634, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP1R7. Positive lysates LY400956 (100ug) and LC400956 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PPP1R7 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PPP1R7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500614)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PPP1R7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500614)

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  Anti-PPP1R7 mouse monoclonal antibody (TA500614) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPP1R7 (RC201634).

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  Immunofluorescent staining of HT29 cells using anti-PPP1R7 mouse monoclonal antibody (TA500614).

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Specifications

Product Data

• Clone Name: OTI4F9
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:50~100
• Reactivities: Human, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PPP1R7 (NP_002703) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 41.4 kDa
• Gene Name: Homo sapiens protein phosphatase 1 regulatory subunit 7 (PPP1R7), transcript variant 1, mRNA.
• Database Link:
  NP_002703
  Entrez Gene 301618 RatEntrez Gene 100855698 DogEntrez Gene 5510 Human
  UniProt ID Q15435
• Synonyms: SDS22

Reference Data

• Protein Families: Druggable Genome, Phosphatase