ALDH2 Mouse Monoclonal Antibody [Clone ID: OTI4H2]

CAT#: CF500615

Carrier-free (BSA/glycerol-free) ALDH2 mouse monoclonal antibody, clone OTI4H2 (formerly 4H2)

Product Images

Anti-ALDH2 mouse monoclonal antibody (TA500615) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ALDH2 (RC200505).

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  Immunofluorescent staining of HepG2 cells using anti-ALDH2 mouse monoclonal antibody (TA500615).

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  HEK293T cells transfected with either RC200505 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ALDH2 antibody (TA500615), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI4H2
• Applications: FC, IF
• Recommended Dilution: IF 1:50~100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG3
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ALDH2 (NP_000681) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.4 kDa
• Gene Name: Homo sapiens aldehyde dehydrogenase 2 family member (ALDH2), transcript variant 1, mRNA; nuclear gene for mitochondrial product.
• Database Link:
  NP_000681
  Entrez Gene 11669 MouseEntrez Gene 29539 RatEntrez Gene 217 Human
  UniProt ID P05091
• Background: This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Synonyms: ALDH-E2; ALDHI; ALDM

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, Ascorbate and aldarate metabolism, beta-Alanine metabolism, Butanoate metabolism, Fatty acid metabolism, Glycerolipid metabolism, Glycolysis / Gluconeogenesis, Histidine metabolism, Limonene and pinene degradation, Lysine degradation, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism, Tryptophan metabolism, Valine, leucine and isoleucine degradation