ERCC1 Mouse Monoclonal Antibody [Clone ID: OTI1A3]

CAT#: CF500622

Carrier-free (BSA/glycerol-free) ERCC1 mouse monoclonal antibody, clone OTI1A3 (formerly 1A3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1. Positive lysates LY419605 (100ug) and LC419605 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500622, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500622, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500622, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500622, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500622, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500622, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500622, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded thyroid tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500622, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500622, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500622, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500622, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500622, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded bladder tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500622, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500622, Dilution 1:50)

• Anti-ERCC1 mouse monoclonal antibody (TA500622) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1 (RC200478).

• HEK293T cells transfected with either pCMV6-ENTRY ERCC1 (RC200478) (Red) or empty vector control plasmid (Blue) were immunostained with anti-ERCC1 mouse monoclonal (TA500622, Dilution 1:1,000), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1A3
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100, Flow 1:100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human ERCC1 expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32.6 kDa
• Gene Name: Homo sapiens ERCC excision repair 1, endonuclease non-catalytic subunit (ERCC1), transcript variant 2, mRNA.
• Database Link:
  NP_001974
  Entrez Gene 13870 MouseEntrez Gene 2067 Human
  UniProt ID P07992
• Background: The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand.
• Synonyms: COFS4; RAD10; UV20

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Nucleotide excision repair