PDE10A Mouse Monoclonal Antibody [Clone ID: OTI3G9]

CAT#: CF500638

Carrier-free (BSA/glycerol-free) PDE10A mouse monoclonal antibody, clone OTI3G9 (formerly 3G9)

Product Images

Anti-PDE10A mouse monoclonal antibody (TA500638) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE10A (RC211181).

• 

  HEK293T cells transfected with either RC211181 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDE10A antibody (TA500638), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI3G9
• Applications: FC, IF
• Recommended Dilution: IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PDE10A (NP_006652) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens phosphodiesterase 10A (PDE10A), transcript variant 2, mRNA.
• Database Link:
  NP_006652
  Entrez Gene 63885 RatEntrez Gene 10846 Human
  UniProt ID Q9Y233
• Background: Various cellular responses are regulated by the second messengers cAMP and cGMP. Phosphodiesterases, such as PDE10A, eliminate cAMP- and cGMP-mediated intracellular signaling by hydrolyzing the cyclic nucleotide to the corresponding nucleoside 5-prime monophosphate (Fujishige et al., 2000 [PubMed 10998054]). [supplied by OMIM]
• Synonyms: FLJ11894; FLJ25677; HSPDE10A

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism