Leukotriene A4 hydrolase (LTA4H) Mouse Monoclonal Antibody [Clone ID: OTI9G8]

CAT#: CF500642

Carrier-free (BSA/glycerol-free) LTA4H mouse monoclonal antibody, clone OTI9G8 (formerly 9G8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LTA4H (RC207617, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LTA4H. Positive lysates LY424467 (100ug) and LC424467 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LTA4H monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-LTA4H mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500642, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-LTA4H mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500642, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-LTA4H mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500642, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-LTA4H mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500642, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-LTA4H mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500642, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-LTA4H mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500642, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-LTA4H mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500642, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-LTA4H mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500642, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-LTA4H mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500642, Dilution 1:50)

• Anti-LTA4H mouse monoclonal antibody (TA500642) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LTA4H (RC207617).

• HEK293T cells transfected with either pCMV6-ENTRY LTA4H (RC207617) (Red) or empty vector control plasmid (Blue) were immunostained with anti-LTA4H mouse monoclonal (TA500642), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI9G8
• Applications: FC, IF, IHC, WB
• Recommended Dilution: IHC 1:100~200, WB: 1:200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human LTA4H (NP_000886) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 69.3 kDa
• Gene Name: Homo sapiens leukotriene A4 hydrolase (LTA4H), transcript variant 1, mRNA.
• Database Link:
  NP_000886
  Entrez Gene 16993 MouseEntrez Gene 299732 RatEntrez Gene 4048 Human
  UniProt ID P09960
• Background: Hydrolyzes an epoxide moiety of leukotriene A4 (LTA-4) to form leukotriene B4 (LTB-4). The enzyme also has some peptidase activity
• Synonyms: leukotriene A4 hydrolase

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways