ILF2 Mouse Monoclonal Antibody [Clone ID: OTI6F1]

CAT#: CF500674

Carrier-free (BSA/glycerol-free) ILF2 mouse monoclonal antibody, clone OTI6F1 (formerly 6F1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ILF2 (RC201751, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ILF2. Positive lysates LY401437 (100ug) and LC401437 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ILF2 monoclonal antibody.

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  Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-ILF2 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-ILF2mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500674, Dilution 1:50)

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  Anti-ILF2 mouse monoclonal antibody (TA500674) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ILF2 (RC201751).

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Specifications

Product Data

• Clone Name: OTI6F1
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:50, IF 1:100
• Reactivities: Human, Mouse, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human ILF2 expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43.1 kDa
• Gene Name: Homo sapiens interleukin enhancer binding factor 2 (ILF2), transcript variant 1, mRNA.
• Database Link:
  NP_004506
  Entrez Gene 67781 MouseEntrez Gene 480139 DogEntrez Gene 3608 Human
  UniProt ID Q12905
• Background: Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2.In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of thisgene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression.
• Synonyms: NF45; PRO3063

Reference Data

• Protein Families: Druggable Genome, Transcription Factors