Peroxiredoxin 4 (PRDX4) Mouse Monoclonal Antibody [Clone ID: OTI9A3]

CAT#: CF500684

Carrier-free (BSA/glycerol-free) PRDX4 mouse monoclonal antibody, clone OTI9A3 (formerly 9A3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRDX4 (RC203330, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRDX4. Positive lysates LY401927 (100ug) and LC401927 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PRDX4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500684)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PRDX4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500684)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-PRDX4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500684)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PRDX4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500684)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PRDX4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500684)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PRDX4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500684)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PRDX4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500684)

• Anti-PRDX4 mouse monoclonal antibody (TA500684) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRDX4 (RC203330).

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Specifications

Product Data

• Clone Name: OTI9A3
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PRDX4 (NP_006397) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.4 kDa
• Gene Name: Homo sapiens peroxiredoxin 4 (PRDX4), mRNA.
• Database Link:
  NP_006397
  Entrez Gene 53381 MouseEntrez Gene 85274 RatEntrez Gene 10549 Human
  UniProt ID Q13162
• Background: The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB. [provided by RefSeq]
• Synonyms: AOE37-2; AOE372; HEL-S-97n; PRX-4

Reference Data

• Protein Families: Druggable Genome