BCKDH kinase (BCKDK) Mouse Monoclonal Antibody [Clone ID: OTI11C9]

CAT#: CF500685

Carrier-free (BSA/glycerol-free) BCKDK mouse monoclonal antibody, clone OTI11C9 (formerly 11C9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCKDK (RC203601, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCKDK. Positive lysates LY401780 (100ug) and LC401780 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-BCKDK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500685)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-BCKDK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500685)

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  Anti-BCKDK mouse monoclonal antibody (TA500685) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BCKDK (RC203601).

Specifications

Product Data

• Clone Name: OTI11C9
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BCKDK (NP_005872) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46.4 kDa
• Gene Name: Homo sapiens branched chain keto acid dehydrogenase kinase (BCKDK), transcript variant 1, mRNA.
• Database Link:
  NP_005872
  Entrez Gene 12041 MouseEntrez Gene 29603 RatEntrez Gene 10295 Human
  UniProt ID O14874
• Background: Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex,the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex .
• Synonyms: BCKDKD; BDK

Reference Data

• Protein Families: Druggable Genome, Protein Kinase