PSMA7 Mouse Monoclonal Antibody [Clone ID: OTI5E3]

CAT#: CF500690

Carrier-free (BSA/glycerol-free) PSMA7 mouse monoclonal antibody, clone OTI5E3 (formerly 5E3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMA7 (RC201169, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMA7. Positive lysates LY400987 (100ug) and LC400987 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either pCMV6-ENTRY PSMA7 (RC201169) (Red) or empty vector control plasmid (Blue) were immunostained with anti-PSMA7 mouse monoclonal (TA500690, Dilution 1:1,000), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI5E3
• Applications: FC, WB
• Recommended Dilution: WB 1:500, IHC 1:50, Flow 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PSMA7 (NP_002783) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 27.9 kDa
• Gene Name: Homo sapiens proteasome subunit alpha 7 (PSMA7), mRNA.
• Database Link:
  NP_002783
  Entrez Gene 26444 MouseEntrez Gene 29674 RatEntrez Gene 5688 Human
  UniProt ID O14818
• Background: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. This particular subunit has been shown to interact specifically with the hepatitis B virus X protein, a protein critical to viral replication. In addition, this subunit is involved in regulating hepatitis virus C internal ribosome entry site (IRES) activity, an activity essential for viral replication. This core alpha subunit is also involved in regulating the hypoxia-inducible factor-1alpha, a transcription factor important for cellular responses to oxygen tension. Multiple isoforms of this subunit arising from alternative splicing may exist but alternative transcripts for only two isoforms have been defined. A pseudogene has been identified on chromosome 9.
• Synonyms: C6; HEL-S-276; HSPC; RC6-1; XAPC7

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Proteasome