Sorbitol Dehydrogenase (SORD) Mouse Monoclonal Antibody [Clone ID: OTI9E7]

CAT#: CF500701

Carrier-free (BSA/glycerol-free) SORD mouse monoclonal antibody, clone OTI9E7 (formerly 9E7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SORD (RC200415, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SORD. Positive lysates LY401082 (100ug) and LC401082 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SORD monoclonal antibody.

• 

  Anti-SORD mouse monoclonal antibody (TA500701) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SORD (RC200415).

• 

  Immunofluorescent staining of HepG2 cells using anti-SORD mouse monoclonal antibody (TA500701).

• 

  HEK293T cells transfected with either pCMV6-ENTRY SORD (RC200415) (Red) or empty vector control plasmid (Blue) were immunostained with anti-SORD mouse monoclonal (TA500701, Dilution 1:1,000), and then analyzed by flow cytometry.

• 

  Flow cytometric analysis of Hela cells, using anti-SORD antibody (TA500701), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).

• Flow cytometric analysis of Jurkat cells, using anti-SORD antibody (TA500701), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).

Show all 7 images

Specifications

Product Data

• Clone Name: OTI9E7
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:1000, IF 1:100, Flow 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SORD (NP_003095) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38.3 kDa
• Gene Name: Homo sapiens sorbitol dehydrogenase (SORD), transcript variant 1, mRNA.
• Database Link:
  NP_003095
  Entrez Gene 6652 Human
  UniProt ID Q00796
• Synonyms: HEL-S-95n; SORD1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism, Metabolic pathways