IBRDC2 (RNF144B) Mouse Monoclonal Antibody [Clone ID: OTI10A12]

CAT#: CF500706

Carrier-free (BSA/glycerol-free) RNF144B mouse monoclonal antibody, clone OTI10A12 (formerly 10A12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF144B (RC209302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF144B. Positive lysates LY405351 (100ug) and LC405351 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI10A12
• Applications: WB
• Recommended Dilution: WB 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-256 of human RNF144B (NP_877434) produced in E. coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.7 kDa
• Gene Name: Homo sapiens ring finger protein 144B (RNF144B), mRNA.
• Database Link:
  NP_877434
  Entrez Gene 218215 MouseEntrez Gene 255488 Human
  UniProt ID Q7Z419
• Background: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a TP53/p53-dependent but caspase-independent mechanism.
• Synonyms: bA528A10.3; IBRDC2; p53RFP; PIR2

Reference Data

• Protein Families: Transmembrane