Prolactin (PRL) Mouse Monoclonal Antibody [Clone ID: OTI6B1]

CAT#: CF500719

Carrier-free (BSA/glycerol-free) PRL mouse monoclonal antibody, clone OTI6B1 (formerly 6B1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRL (RC205627, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRL. Positive lysates LY424443 (100ug) and LC424443 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PRL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500719)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-PRL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500719)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PRL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500719)

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  Anti-PRL mouse monoclonal antibody (TA500719) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRL (RC205627).

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  Immunofluorescent staining of HepG2 cells using anti-PRL mouse monoclonal antibody (TA500719).

• Flow cytometric Analysis of Hela cells, using anti-PRL antibody (TA500719), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-PRL antibody (TA500719), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI6B1
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:50, IF 1:50~100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PRL (NP_000939) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25.7 kDa
• Gene Name: Homo sapiens prolactin (PRL), transcript variant 1, mRNA.
• Database Link:
  NP_000939
  Entrez Gene 5617 Human
  UniProt ID P01236
• Synonyms: GHA1

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, Neuroactive ligand-receptor interaction