Prolactin (PRL) Mouse Monoclonal Antibody [Clone ID: OTI2B7]

CAT#: CF500721

Carrier-free (BSA/glycerol-free) PRL mouse monoclonal antibody, clone OTI2B7 (formerly 2B7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRL (RC205627, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRL. Positive lysates LY424443 (100ug) and LC424443 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2B7
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PRL (NP_000939) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25.9 kDa
• Gene Name: Homo sapiens prolactin (PRL), transcript variant 1, mRNA.
• Database Link:
  NP_000939
  Entrez Gene 5617 Human
  UniProt ID P01236
• Background: Prolactin acts primarily on the mammary gland by promoting lactation
• Synonyms: GHA1

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, Neuroactive ligand-receptor interaction