IBRDC2 (RNF144B) Mouse Monoclonal Antibody [Clone ID: OTI6B11]

CAT#: CF500722

Carrier-free (BSA/glycerol-free) RNF144B mouse monoclonal antibody, clone OTI6B11 (formerly 6B11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF144B (RC209302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF144B. Positive lysates LY405351 (100ug) and LC405351 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RNF144B monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Anti-RNF144B mouse monoclonal antibody (TA500722) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B (RC209302 ).

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  HEK293T cells transfected with either RC209302 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RNF144B antibody (TA500722), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-RNF144B antibody (TA500722), (Red), compared to a nonspecific negative control antibody, (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-RNF144B antibody (TA500722), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI6B11
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RNF144B (NP_877434) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.5 kDa
• Gene Name: Homo sapiens ring finger protein 144B (RNF144B), mRNA.
• Database Link:
  NP_877434
  Entrez Gene 218215 MouseEntrez Gene 255488 Human
  UniProt ID Q7Z419
• Synonyms: bA528A10.3; IBRDC2; p53RFP; PIR2

Reference Data

• Protein Families: Transmembrane