BTN3A2 Mouse Monoclonal Antibody [Clone ID: OTI1A6]

CAT#: CF500730

Carrier-free (BSA/glycerol-free) BTN3A2 mouse monoclonal antibody, clone OTI1A6 (formerly 1A6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY BTN3A2 (Cat# RC201183, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTN3A2(Cat# TA500730). Positive lysates LY402082 (100ug) and LC402082 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Anti-BTN3A2 mouse monoclonal antibody (TA500730) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTN3A2 (RC201183).

• Immunofluorescent staining of HT29 cells using anti-BTN3A2 mouse monoclonal antibody (TA500730).

• Figure from citation: Vg2Vd2 T cells proliferate in response to the 20.1 but not the 1A6 mAb, although both are specific for BTN3. The 12G12 Vg2Vd2 T cell clone was cultured with control IgG1 mAb, BTN3A2 (1A6) mAb, or 20.1 mAb in the presence of mitomycin C–treated Va2 cells. The cells were pulsed with [3H]thymidine after 24 h and harvested 18 h later. View Citation

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Specifications

Product Data

• Clone Name: OTI1A6
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BTN3A2 (NP_008978) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36.4 kDa
• Gene Name: butyrophilin subfamily 3 member A2
• Database Link:
  NP_008978
  Entrez Gene 11118 Human
  UniProt ID P78410
• Background: This gene encodes a member of the immunoglobulin superfamily, containing two Ig domains with similarity to Ig variable and Ig constant domains. The gene resides in the juxta-telomeric region of the major histocompatability class 1 locus on chromosome 6 in the seven member BTN cluster, which includes butyrophilin, and three members each of the BTN2 and BTN3 subfamilies. Alternatively spliced transcript variants have been described but their full-length nature has yet to be determined.
• Synonyms: BT3.2; BTF4; BTN3.2; CD277

Reference Data

• Protein Families: Druggable Genome, Transmembrane