CD26 (DPP4) Mouse Monoclonal Antibody [Clone ID: OTI11D7]

CAT#: CF500733

Carrier-free (BSA/glycerol-free) DPP4 mouse monoclonal antibody, clone OTI11D7 (formerly 11D7)

Product Images

Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human DPP4 plasmid (RC209466, lane 2), mouse DPP4 plasmid (MR222698, lane 3) using anti-DPP4 antibody TA500733 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

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  Western blot analysis of extracts (50ug per lane) from 2 cell lines lysates by using anti-DPP4 monoclonal antibody(TA500733, 1:500)

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  Figure from citation: Western blot analysis of protein levels of DPP4/8/9 in 293E cells transfected with pcDNA3.1 (Mock), pcDNA3.1-hDPP8 (DPP8) and pcDNA3.1-hDPP9 (DPP9), the purified recombinant human DPP4 protein (rhDPP4) was used as a positive control for the DPP4 antibody, antibodies targeting human DPP4/8/9 (TA500733/TA504366/TA504040) and β-actin (TA310155) were purchased from OriGene. View Citation

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  Figure from citation: Western Blot of DPP4 protein level by using anti-DPP4 antibody in mouse MLE15 cells. View Citation

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  Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-DPP4mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Figure from citation: Immunohistochemistry of DPP4 protein level by using anti-DPP4 antibody in lungs harvested from BALB/c mice at day 3. Dilution: 1:800 View Citation. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded normal human thyroid tissue using anti-DPP4 C/N TA500733 clone OTI1D7 mouse monoclonal antibody. HIER Citrate buffer pH6 ([B05-100]) at 110C for 10 min, anti-DPP4 diluted to 1:200. Detection was done with Polink1 Broad Mouse and Rabbit C/N D11-18 with DAB Kit. Image on left is at 10x magnification and image on the right is at 40x magnification of area indicated by red arrow on 10x. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded normal human kidney tissue using anti-DPP4 C/N TA500733 clone OTI1D7 mouse monoclonal antibody. HIER Citrate buffer pH6 ([B05-100]) at 110C for 10 min, anti-DPP4 diluted to 1:100. Detection was done with Polink1 Broad Mouse and Rabbit C/N D11-18 with DAB Kit. Image on left is at 10x magnification and image on the right is at 40x magnificationof area indicated by red arrow on 10x. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Anti-DPP4 mouse monoclonal antibody (TA500733) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DPP4 (RC209466).

• Flow cytometric analysis of Hela cells, using anti-DPP4 antibody (TA500733), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).

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Specifications

Product Data

• Clone Name: OTI11D7
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:50, IF 1:100, Flow 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DPP4 (NP_001926) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 88.3 kDa
• Gene Name: dipeptidyl peptidase 4
• Database Link:
  NP_001926
  Entrez Gene 1803 Human
  UniProt ID P27487
• Background: The protein encoded by this gene is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides.
• Synonyms: ADABP; ADCP2; CD26; DPPIV; TP103

Reference Data

• Protein Families: Druggable Genome, Protease, Secreted Protein, Transmembrane