IDH3A Mouse Monoclonal Antibody [Clone ID: OTI2F11]

CAT#: CF500739

Carrier-free (BSA/glycerol-free) IDH3A mouse monoclonal antibody, clone OTI2F11 (formerly 2F11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDH3A (RC200313, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDH3A. Positive lysates LY401698 (100ug) and LC401698 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IDH3A monoclonal antibody.

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  Figure from citation: Western blot analysis of IDH3A protein level by using anti-IDH3A antibody in fibroblasts with miR-424 knockdown or overexpression, and in TGF-b1-treated fibroblasts with or without miR-424 depletion. View Citation

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  Anti-IDH3A mouse monoclonal antibody (TA500739) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IDH3A (RC200313).

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  Immunofluorescent staining of HepG2 cells using anti-IDH3A mouse monoclonal antibody (TA500739).

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  Flow cytometric analysis of Hela cells, using anti-IDH3A antibody (TA500739), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).

• Flow cytometric analysis of Jurkat cells, using anti-IDH3A antibody (TA500739), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).

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Specifications

Product Data

• Clone Name: OTI2F11
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, Flow 1:100
• Reactivities: Human, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human IDH3A (NP_005521) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 39.6 kDa
• Gene Name: Homo sapiens isocitrate dehydrogenase (NAD(+)) 3 alpha (IDH3A), mRNA.
• Database Link:
  NP_005521
  Entrez Gene 67834 MouseEntrez Gene 114096 RatEntrez Gene 479066 DogEntrez Gene 3419 Human
  UniProt ID P50213
• Background: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.
• Synonyms: H-IDH alpha; isocitrate dehydrogenase (NAD+) alpha chain; isocitrate dehydrogenase 3 (NAD+) a; isocitrate dehydrogenase [NAD] subunit alpha; isocitric dehydrogenase; mitochondrial; NAD(H)-specific isocitrate dehydrogenase alpha subunit; NAD+-specific ICDH

Reference Data

• Protein Pathways: Citrate cycle (TCA cycle), Metabolic pathways