PPME1 Mouse Monoclonal Antibody [Clone ID: OTI2C5]

CAT#: CF500768

Carrier-free (BSA/glycerol-free) PPME1 mouse monoclonal antibody, clone OTI2C5 (formerly 2C5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPME1 (RC200009, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPME1. Positive lysates LY402509 (100ug) and LC402509 (20ug) can be purchased separately from OriGene.

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  Anti-PPME1 mouse monoclonal antibody (TA500768) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPME1 (RC200009).

Specifications

Product Data

• Clone Name: OTI2C5
• Applications: IF, WB
• Recommended Dilution: WB 1:1000, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PPME1 (NP_057231) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42.3 kDa
• Gene Name: Homo sapiens protein phosphatase methylesterase 1 (PPME1), transcript variant 1, mRNA.
• Database Link:
  NP_057231
  Entrez Gene 72590 MouseEntrez Gene 361613 RatEntrez Gene 51400 Human
  UniProt ID Q9Y570
• Background: Protein phosphatase methylesterase-1 catalyzes the demethylation of the protein phosphatase-2A catalytic subunit
• Synonyms: PME-1

Reference Data

• Protein Families: Druggable Genome