TTLL12 Mouse Monoclonal Antibody [Clone ID: OTI1A2]

CAT#: CF500777

Carrier-free (BSA/glycerol-free) TTLL12 mouse monoclonal antibody, clone OTI1A2 (formerly 1A2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TTLL12 (RC200841, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TTLL12. Positive lysates LY402417 (100ug) and LC402417 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-TTLL12 monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-TTLL12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500777, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-TTLL12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500777, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-TTLL12mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500777, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-TTLL12mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500777, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-TTLL12mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500777, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-TTLL12mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500777, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-TTLL12mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500777, Dilution 1:50)

• HEK293T cells transfected with either pCMV6-ENTRY TTLL12 (RC200841) (Red) or empty vector control plasmid (Blue) were immunostained with anti-TTLL12 mouse monoclonal (TA500777), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1A2
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, Flow 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TTLL12 (NP_055955) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 74.4 kDa
• Gene Name: Homo sapiens tubulin tyrosine ligase like 12 (TTLL12), mRNA.
• Database Link:
  NP_055955
  Entrez Gene 300105 RatEntrez Gene 23170 Human
  UniProt ID Q14166
• Synonyms: dJ526I14.2