ID3 Mouse Monoclonal Antibody [Clone ID: OTI10D3]

CAT#: CF500791

Carrier-free (BSA/glycerol-free) ID3 mouse monoclonal antibody, clone OTI10D3 (formerly 10D3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ID3 (RC200583, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ID3. Positive lysates LY419492 (100ug) and LC419492 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-ID3 monoclonal antibody at 1:200 dilution.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ID3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500791)

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  Flow cytometric Analysis of Hela cells, using anti-ID3 antibody (TA500791), (Red), compared to a nonspecific negative control antibody, (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-ID3 antibody (TA500791), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI10D3
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:50, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ID3 (NP_002158) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 12.8 kDa
• Gene Name: Homo sapiens inhibitor of DNA binding 3, HLH protein (ID3), mRNA.
• Database Link:
  NP_002158
  Entrez Gene 3399 Human
  UniProt ID Q02535
• Background: Members of the ID family of helix-loop-helix (HLH) proteins lack a basic DNA-binding domain and inhibit transcription through formation of nonfunctional dimers that are incapable of binding to DNA. [supplied by OMIM]
• Synonyms: bHLHb25; HEIR-1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: TGF-beta signaling pathway