MAN1 (LEMD3) Mouse Monoclonal Antibody [Clone ID: OTI4E1]

CAT#: CF500797

Carrier-free (BSA/glycerol-free) LEMD3 mouse monoclonal antibody, clone OTI4E1 (formerly 4E1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LEMD3 (RC212759, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LEMD3. Positive lysates LY402311 (100ug) and LC402311 (20ug) can be purchased separately from OriGene.

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  Flow cytometric analysis of Jurkat cells, using anti-LEMD3 antibody (TA500797), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).

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  Flow cytometric analysis of Hela cells, using anti-LEMD3 antibody (TA500797), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).

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  Anti-LEMD3 mouse monoclonal antibody (TA500797) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LEMD3 (RC212759).

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  Immunoprecipitation of LEMD3 by using TrueMab monoclonal anti-LEMD3 antibody (Negative control: IP without adding anti-LEMD3 antibody). For each experiment, 500ul of DDK tagged LEMD3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-LEMD3 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

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Specifications

Product Data

• Clone Name: OTI4E1
• Applications: IP, WB
• Recommended Dilution: WB 1:1000, IF 1:100, Flow 1:100, IP 2-4ug/mg
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human LEMD3 (NP_055134) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 100.0 kDa
• Gene Name: Homo sapiens LEM domain containing 3 (LEMD3), transcript variant 1, mRNA.
• Database Link:
  NP_055134
  Entrez Gene 380664 MouseEntrez Gene 680066 RatEntrez Gene 23592 Human
  UniProt ID Q9Y2U8
• Background: This locus encodes a LEM domain-containing protein. The encoded protein functions to antagonize transforming growth factor-beta signaling at the inner nuclear membrane. Two transcript variants encoding different isoforms have been found for this gene. Mutations in this gene have been associated with osteopoikilosis, Buschke-Ollendorff syndrome and melorheostosis
• Synonyms: MAN1

Reference Data

• Protein Families: Transmembrane