AKT2 Mouse Monoclonal Antibody [Clone ID: OTI4H7]

CAT#: CF500814

Carrier-free (BSA/glycerol-free) AKT2 mouse monoclonal antibody, clone OTI4H7 (formerly 4H7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT2 (RC217733, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT2. Positive lysates LY419836 (100ug) and LC419836 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-AKT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500814)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-AKT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500814)

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  Flow cytometric Analysis of Jurkat cells, using anti-AKT2 antibody (TA500814), (Red), compared to a nonspecific negative control antibody, (Blue).

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  Flow cytometric Analysis of Hela cells, using anti-AKT2 antibody (TA500814), (Red), compared to a nonspecific negative control antibody, (Blue).

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  Anti-AKT2 mouse monoclonal antibody (TA500814) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT2 (RC217733).

• Immunofluorescent staining of HepG2 cells using anti-AKT2 mouse monoclonal antibody (TA500814).

• Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-AKT2 monoclonal antibodyat 1:200 dilution.

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Specifications

Product Data

• Clone Name: OTI4H7
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:50, IF 1:50~100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human AKT2 (NP_001617) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 55.6 kDa
• Gene Name: Homo sapiens AKT serine/threonine kinase 2 (AKT2), transcript variant 1, mRNA.
• Database Link:
  NP_001617
  Entrez Gene 11652 MouseEntrez Gene 25233 RatEntrez Gene 208 Human
  UniProt ID P31751
• Background: This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq]
• Synonyms: HIHGHH; PKBB; PKBBETA; PRKBB; RAC-BETA

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Insulin signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Melanoma, mTOR signaling pathway, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Tight junction, Toll-like receptor signaling pathway, VEGF signaling pathway