Aconitase 2 (ACO2) Mouse Monoclonal Antibody [Clone ID: OTI3G8]

CAT#: CF500824

Carrier-free (BSA/glycerol-free) ACO2 mouse monoclonal antibody, clone OTI3G8 (formerly 3G8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACO2 (RC204307, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACO2. Positive lysates LY400442 (100ug) and LC400442 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-ACO2mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500824, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-ACO2mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500824, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-ACO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500824, Dilution 1:50)

• 

  Anti-ACO2 mouse monoclonal antibody (TA500824) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACO2 (RC204307).

• 

  Immunofluorescent staining of HepG2 cells using anti-ACO2 mouse monoclonal antibody (TA500824).

• Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-ACO2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Show all 7 images

Specifications

Product Data

• Clone Name: OTI3G8
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ACO2 (NP_001089) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 85.4 kDa
• Gene Name: Homo sapiens aconitase 2 (ACO2), mRNA.
• Database Link:
  NP_001089
  Entrez Gene 11429 MouseEntrez Gene 79250 RatEntrez Gene 50 Human
  UniProt ID Q99798
• Background: The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.
• Synonyms: ACONM; HEL-S-284; ICRD; OCA8; OPA9

Reference Data

• Protein Pathways: Citrate cycle (TCA cycle), Glyoxylate and dicarboxylate metabolism, Metabolic pathways