PASK Mouse Monoclonal Antibody [Clone ID: OTI8G9]

CAT#: CF500826

Carrier-free (BSA/glycerol-free) PASK mouse monoclonal antibody, clone OTI8G9 (formerly 8G9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PASK (RC209096, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PASK. Positive lysates LY414744 (100ug) and LC414744 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PASK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500826)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PASK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500826)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PASK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500826)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PASK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500826)

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  Anti-PASK mouse monoclonal antibody (TA500826) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PASK (RC209096).

• Flow cytometric Analysis of Hela cells, using anti-PASK antibody (TA500826), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-PASK antibody (TA500826), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI8G9
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PASK (NP_055963) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 142.7 kDa
• Gene Name: Homo sapiens PAS domain containing serine/threonine kinase (PASK), transcript variant 2, mRNA.
• Database Link:
  NP_055963
  Entrez Gene 23178 Human
  UniProt ID Q96RG2
• Background: PAS domains regulate the function of many intracellular signaling pathways in response to both extrinsic and intrinsic stimuli. PASK is an evolutionarily conserved protein present in yeast, flies, and mammals. [supplied by OMIM]
• Synonyms: PASKIN; STK37

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency