ATP5F1B Mouse Monoclonal Antibody [Clone ID: OTI6B11]

CAT#: CF500850

Carrier-free (BSA/glycerol-free) ATP5B mouse monoclonal antibody, clone OTI6B11 (formerly 6B11)

Formulation: Standard Carrier Free


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USD 465.00

In Stock*

Size
    • 100 ug

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Specifications

Product Data
Clone Name OTI6B11
Applications IHC, WB
Recommended Dilution WB 1:2000, IHC 1:50
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Full length human recombinant protein of human ATP5B (NP_001677) produced in HEK293T cell.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 56.6 kDa
Gene Name Homo sapiens ATP synthase F1 subunit beta (ATP5F1B), mRNA.
Background This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis,utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.
Synonyms ATPMB; ATPSB; HEL-S-271
Reference Data
Protein Families Druggable Genome
Protein Pathways Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease

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