TIPRL Mouse Monoclonal Antibody [Clone ID: OTI3D11]

CAT#: CF500853

Carrier-free (BSA/glycerol-free) TIPRL mouse monoclonal antibody, clone OTI3D11 (formerly 3D11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TIPRL (RC223912, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TIPRL. Positive lysates LY403496 (100ug) and LC403496 (20ug) can be purchased separately from OriGene.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HEK293T cells (WT, Cat# LC810293T) and TIPRL-Knockout HEK293T cells (KO, Cat# LC842492) were separated by SDS-PAGE and immunoblotted with anti-TIPRL monoclonal antibody TA500853 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  Flow cytometric analysis of Hela cells, using anti-TIPRL antibody (TA500853), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).

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  Flow cytometric analysis of Jurkat cells, using anti-TIPRL antibody (TA500853), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).

Specifications

Product Data

• Clone Name: OTI3D11
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, Flow 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TIPRL (NP_690866) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 31.4 kDa
• Gene Name: Homo sapiens TOR signaling pathway regulator (TIPRL), transcript variant 1, mRNA.
• Database Link:
  NP_690866
  Entrez Gene 261726 Human
  UniProt ID O75663
• Background: TIPRL is an inhibitory regulator of protein phosphatase-2A , PP4 , and PP6
• Synonyms: TIP; TIP41