GRASP1 (GRIPAP1) Mouse Monoclonal Antibody [Clone ID: OTI2C11]

CAT#: CF500855

Carrier-free (BSA/glycerol-free) GRIPAP1 mouse monoclonal antibody, clone OTI2C11 (formerly 2C11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRIPAP1 (RC210936, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRIPAP1. Positive lysates LY402754 (100ug) and LC402754 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-GRIPAP1 monoclonal antibody (1:200).

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  Anti-GRIPAP1 mouse monoclonal antibody (TA500855) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GRIPAP1 (RC210936).

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  HEK293T cells transfected with either pCMV6-ENTRY GRIPAP1 (RC210936) (Red) or empty vector control plasmid (Blue) were immunostained with anti-GRIPAP1 mouse monoclonal (TA500855), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI2C11
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:1000, IF 1:100, Flow 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GRIPAP1 (NP_064522) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 96.0 kDa
• Gene Name: Homo sapiens GRIP1 associated protein 1 (GRIPAP1), mRNA.
• Database Link:
  NP_064522
  Entrez Gene 54645 MouseEntrez Gene 116493 RatEntrez Gene 56850 Human
  UniProt ID Q4V328
• Background: This gene encodes a guanine nucleotide exchange factor for the Ras family of small G proteins (RasGEF). In brain studies, the encoded protein was found with the GRIP/AMPA receptor complex. Multiple alternatively spliced transcript variants have been described that encode different protein isoforms; however, the full-length nature and biological validity of all of these variants have not been determined
• Synonyms: GRASP-1

Reference Data

• Protein Families: Druggable Genome