ERMAP Mouse Monoclonal Antibody [Clone ID: OTIC8]

CAT#: CF500911

Carrier-free (BSA/glycerol-free) anti-ERMAP mouse monoclonal antibody, clone OTIC8 (formerly C8)

Product Images

Anti-ERMAP mouse monoclonal antibody (TA500911) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERMAP (RC215022).

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  HEK293T cells transfected with either pCMV6-ENTRY ERMAP (RC215022) (Red) or empty vector control plasmid (Blue) were immunostained with anti-ERMAP mouse monoclonal (TA500911), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of living K562 cells, using anti-ERMAP antibody (TA500911), (Red), compared to a nonspecific negative control antibody, (Blue).

Specifications

Product Data

• Clone Name: OTIC8
• Applications: FC, IF
• Recommended Dilution: IF: 1:100, Flow: 1:50-1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ERMAP (NP_061008) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52.6 kDa
• Gene Name: Homo sapiens erythroblast membrane associated protein (Scianna blood group) (ERMAP), transcript variant 1, mRNA.
• Database Link:
  NP_001017922
  Entrez Gene 114625 Human
  UniProt ID Q96PL5
• Background: The protein encoded by this gene is a cell surface transmembrane protein that may act as an erythroid cell receptor,possibly as a mediator of cell adhesion. Polymorphisms in this gene are responsible for the Scianna/Radin blood group system. Two transcript variants encoding the same protein have been found for this gene.
• Synonyms: BTN5; PRO2801; RD; SC

Reference Data

• Protein Families: Druggable Genome, Transmembrane