HIPPI (IFT57) Mouse Monoclonal Antibody [Clone ID: OTI4F6]

CAT#: CF500918

Carrier-free (BSA/glycerol-free) IFT57 mouse monoclonal antibody, clone OTI4F6 (formerly 4F6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY IFT57 (Cat# RC204116, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFT57 (Cat# TA500918). Positive lysates LY402637 (100ug) and LC402637 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IFT57 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

• 

  Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-IFT57 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-IFT57mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• 

  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-IFT57mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-IFT57mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Anti-IFT57 mouse monoclonal antibody (TA500918) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IFT57 (RC204116).

Show all 7 images

Specifications

Product Data

• Clone Name: OTI4F6
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human IFT57(NP_060480) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49.1 kDa
• Gene Name: intraflagellar transport 57
• Database Link:
  NP_060480
  Entrez Gene 73916 MouseEntrez Gene 303968 RatEntrez Gene 55081 Human
  UniProt ID Q9NWB7
• Background: Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1,leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3'present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear
• Synonyms: ESRRBL1; HIPPI; MHS4R2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Huntington's disease