CISD1 Mouse Monoclonal Antibody [Clone ID: OTI4G3]

CAT#: CF500927

Carrier-free (BSA/glycerol-free) CISD1 mouse monoclonal antibody, clone OTI4G3 (formerly 4G3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CISD1 (RC203308, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CISD1. Positive lysates LY413042 (100ug) and LC413042 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-CISD1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500927, Dilution 1:50)

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  Anti-CISD1 mouse monoclonal antibody (TA500927) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CISD1 (RC203308).

Specifications

Product Data

• Clone Name: OTI4G3
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CISD1 (NP_060934) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 12.2 kDa
• Gene Name: Homo sapiens CDGSH iron sulfur domain 1 (CISD1), mRNA.
• Database Link:
  NP_060934
  Entrez Gene 52637 MouseEntrez Gene 294362 RatEntrez Gene 55847 Human
  UniProt ID Q9NZ45
• Background: CISD1 is a member of the CDGSH domain-containing family and may play a role in the regulation of mitochondrial oxidative capacity
• Synonyms: C10orf70; MDS029; mitoNEET; ZCD1

Reference Data

• Protein Families: Transmembrane