PSMC3 Mouse Monoclonal Antibody [Clone ID: OTI10D12]

CAT#: CF500933

Carrier-free (BSA/glycerol-free) PSMC3 mouse monoclonal antibody, clone OTI10D12 (formerly 10D12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMC3 (RC201790, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMC3. Positive lysates LY400992 (100ug) and LC400992 (20ug) can be purchased separately from OriGene.

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  Anti-PSMC3 mouse monoclonal antibody (TA500933) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMC3 (RC201790).

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  HEK293T cells transfected with either pCMV6-ENTRY PSMC3 (RC201790) (Red) or empty vector control plasmid (Blue) were immunostained with anti-PSMC3 mouse monoclonal (TA500933), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI10D12
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, Flow 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PSMC3(NP_002795) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49.2 kDa
• Gene Name: Homo sapiens proteasome 26S subunit, ATPase 3 (PSMC3), mRNA.
• Database Link:
  NP_002795
  Entrez Gene 19182 MouseEntrez Gene 29677 RatEntrez Gene 5702 Human
  UniProt ID P17980
• Background: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits.Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9.
• Synonyms: TBP1

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Proteasome