FAHD2A Mouse Monoclonal Antibody [Clone ID: OTI9D4]

CAT#: CF500944

Carrier-free (BSA/glycerol-free) FAHD2A mouse monoclonal antibody, clone OTI9D4 (formerly 9D4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAHD2A (RC211128, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAHD2A. Positive lysates LY402492 (100ug) and LC402492 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-FAHD2A monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-FAHD2Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500944, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-FAHD2Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500944, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-FAHD2Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500944, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-FAHD2Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500944, Dilution 1:50)

• HEK293T cells transfected with either pCMV6-ENTRY FAHD2A (RC211128) (Red) or empty vector control plasmid (Blue) were immunostained with anti-FAHD2A mouse monoclonal (TA500944), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI9D4
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, Flow 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FAHD2A (NP_057128) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34.6 kDa
• Gene Name: Homo sapiens fumarylacetoacetate hydrolase domain containing 2A (FAHD2A), mRNA.
• Database Link:
  NP_057128
  Entrez Gene 51011 Human
  UniProt ID Q96GK7
• Background: May have hydrolase activity
• Synonyms: CGI-105