Annexin A11 (ANXA11) Mouse Monoclonal Antibody [Clone ID: OTI1C6]

CAT#: CF500950

Carrier-free (BSA/glycerol-free) ANXA11 mouse monoclonal antibody, clone OTI1C6 (formerly 1C6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANXA11 (RC212191, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANXA11. Positive lysates LY420097 (100ug) and LC420097 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-ANXA11 monoclonal antibody (1:200).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and ANXA11-Knockout 293T cells (KO, Cat# LC842105) were separated by SDS-PAGE and immunoblotted with anti-ANXA11 monoclonal antibody TA500950 (1:500`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  Anti-ANXA11 mouse monoclonal antibody (TA500950) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ANXA11 (RC212191).

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  HEK293T cells transfected with either pCMV6-ENTRY ANXA11 (RC212191) (Red) or empty vector control plasmid (Blue) were immunostained with anti-ANXA11 mouse monoclonal (TA500950), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1C6
• Applications: FC, IF, WB
• Recommended Dilution: IHC 1:100~200, WB: 1:200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ANXA11 (NP_001148) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.4 kDa
• Gene Name: Homo sapiens annexin A11 (ANXA11), transcript variant a, mRNA.
• Database Link:
  NP_001148
  Entrez Gene 11744 MouseEntrez Gene 290527 RatEntrez Gene 311 Human
  UniProt ID P50995
• Background: This gene encodes a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and conserved C-terminal domains, which contain the calcium-dependent phospholipid-binding sites. The encoded protein is a 56-kD antigen recognized by sera from patients with various autoimmune diseases. Transcript variants encoding the same isoform have been identified.
• Synonyms: ANX11; CAP50