Natriuretic Peptide Receptor C (NPR3) Mouse Monoclonal Antibody [Clone ID: OTI2C2]

CAT#: CF500955

Carrier-free (BSA/glycerol-free) NPR3 mouse monoclonal antibody, clone OTI2C2 (formerly 2C2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NPR3 (RC219453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NPR3. Positive lysates LY424462 (100ug) and LC424462 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-NPR3 monoclonal antibody at 1:200.

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-NPR3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500955, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-NPR3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500955, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-NPR3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500955, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-NPR3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500955, Dilution 1:50)

• Anti-NPR3 mouse monoclonal antibody (TA500955) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NPR3 (RC219453).

• HEK293T cells transfected with either pCMV6-ENTRY NPR3 (RC219453) (Red) or empty vector control plasmid (Blue) were immunostained with anti-NPR3 mouse monoclonal (TA500955), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI2C2
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:200 - 1:1000, IHC 1:50, IF 1:100, Flow 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NPR3 (NP_000899) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 59.8 kDa
• Gene Name: Homo sapiens natriuretic peptide receptor 3 (NPR3), transcript variant 2, mRNA.
• Database Link:
  NP_000899
  Entrez Gene 18162 MouseEntrez Gene 25339 RatEntrez Gene 4883 Human
  UniProt ID P17342
• Background: The family of natriuretic peptides (see MIM 108780) elicit a number of vascular, renal, and endocrine effects that are important in the maintenance of blood pressure and extracellular fluid volume. These effects are mediated by specific binding of the peptides to cell surface receptors in the vasculature, kidney, adrenal, and brain
• Synonyms: ANP-C; ANPR-C; ANPRC; C5orf23; GUCY2B; NPR-C; NPRC

Reference Data

• Protein Families: Druggable Genome, Transmembrane