Annexin A1 (ANXA1) Mouse Monoclonal Antibody [Clone ID: OTI3A8]

CAT#: CF500967

Carrier-free (BSA/glycerol-free) ANXA1 mouse monoclonal antibody, clone OTI3A8 (formerly 3A8)

Product Images

Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and ANXA1-Knockout hela cells (KO, Cat# LC810011) were separated by SDS-PAGE and immunoblotted with anti-ANXA1 monoclonal antibody TA500967. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).

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  Anti-ANXA1 mouse monoclonal antibody (TA500967) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ANXA1 (RC201569).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANXA1 (RC201569, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANXA1. Positive lysates LY424560 (100ug) and LC424560 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ANXA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500967, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-ANXA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500967, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-ANXA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500967, Dilution 1:50)

• Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ANXA1 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)

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Specifications

Product Data

• Clone Name: OTI3A8
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ANXA1 (NP_000691) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38.5 kDa
• Gene Name: Homo sapiens annexin A1 (ANXA1), mRNA.
• Database Link:
  NP_000691
  Entrez Gene 16952 MouseEntrez Gene 25380 RatEntrez Gene 301 Human
  UniProt ID P04083
• Background: Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity.
• Synonyms: ANX1; LPC1

Reference Data

• Protein Families: Druggable Genome