ATP citrate lyase (ACLY) Mouse Monoclonal Antibody [Clone ID: OTI3G8]

CAT#: CF501031

Carrier-free (BSA/glycerol-free) ACLY mouse monoclonal antibody, clone OTI3G8 (formerly 3G8)

Product Images

Anti-ACLY mouse monoclonal antibody (TA501031) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACLY (RC200508).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ACLY (Cat# RC200508, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACLY(Cat# TA501031). Positive lysates LY420122 (100ug) and LC420122 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded bladder tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501031, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501031, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501031, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501031, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501031, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501031, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501031, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501031, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501031, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501031, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501031, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501031, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501031, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501031, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501031, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501031, Dilution 1:50)

• Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-ACLY monoclonal antibody (1:200).

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Specifications

Product Data

• Clone Name: OTI3G8
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ACLY (NP_001087) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 120.8 kDa
• Gene Name: ATP citrate lyase
• Database Link:
  NP_001087
  Entrez Gene 24159 RatEntrez Gene 104112 MouseEntrez Gene 47 Human
  UniProt ID P53396
• Background: ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene.
• Synonyms: ACL; ATPCL; CLATP

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Citrate cycle (TCA cycle), Metabolic pathways