KCTD5 Mouse Monoclonal Antibody [Clone ID: OTI3C8]

CAT#: CF501035

Carrier-free (BSA/glycerol-free) KCTD5 mouse monoclonal antibody, clone OTI3C8 (formerly 3C8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KCTD5 (RC200180, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KCTD5. Positive lysates LY412830 (100ug) and LC412830 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KCTD5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-KCTD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501035, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-KCTD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501035, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-KCTD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501035, Dilution 1:50)

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  Anti-KCTD5 mouse monoclonal antibody (TA501035) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KCTD5 (RC200180).

• Immunofluorescent staining of HeLa cells using anti-KCTD5 mouse monoclonal antibody (TA501035).

• HEK293T cells transfected with either pCMV6-ENTRY KCTD5 (RC200180) (Red) or empty vector control plasmid (Blue) were immunostained with anti-KCTD5 mouse monoclonal (TA501035), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI3C8
• Applications: FC, IF, IHC, WB
• Recommended Dilution: IHC 1:100~400, WB: 1:200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human KCTD5 (NP_061865) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 26.1 kDa
• Gene Name: Homo sapiens potassium channel tetramerization domain containing 5 (KCTD5), mRNA.
• Database Link:
  NP_061865
  Entrez Gene 54442 Human
  UniProt ID Q9NXV2
• Background: Its interaction with CUL3 suggests that it may act as a substrate adapter in some E3 ligase complex. Does not affect the function of Kv channel Kv2.1/KCNB1, Kv1.2/KCNA2, Kv4.2/KCND2 and Kv3.4/KCNC4
• Synonyms: FLJ20040

Reference Data

• Protein Families: Ion Channels: Other