Amyloid Precursor Protein (APP) Mouse Monoclonal Antibody [Clone ID: OTI3E3]

CAT#: CF501042

Carrier-free (BSA/glycerol-free) APP mouse monoclonal antibody, clone OTI3E3 (formerly 3E3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APP (RC221339, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APP. Positive lysates LY424694 (100ug) and LC424694 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-APP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501042, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-APP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501042, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-APP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501042, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-APP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501042, Dilution 1:50)

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  Anti-APP mouse monoclonal antibody (TA501042) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY APP (RC221339).

• HEK293T cells transfected with either pCMV6-ENTRY APP (RC221339) (Red) or empty vector control plasmid (Blue) were immunostained with anti-APP mouse monoclonal (TA501042), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI3E3
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100, Flow 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human APP (NP_000475) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 87 kDa
• Gene Name: Homo sapiens amyloid beta precursor protein (APP), transcript variant 1, mRNA.
• Database Link:
  NP_000475
  Entrez Gene 11820 MouseEntrez Gene 54226 RatEntrez Gene 351 Human
  UniProt ID P05067
• Background: This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene.
• Synonyms: AAA; ABETA; ABPP; AD1; APPI; CTFgamma; CVAP; PN-II; PN2

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Alzheimer's disease