TPMT Mouse Monoclonal Antibody [Clone ID: OTI4B1]

CAT#: CF501069

Carrier-free (BSA/glycerol-free) TPMT mouse monoclonal antibody, clone OTI4B1 (formerly 4B1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TPMT (RC203309, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TPMT. Positive lysates LY400131 (100ug) and LC400131 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-TPMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501069, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-TPMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501069, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-TPMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501069, Dilution 1:50)

Specifications

Product Data

• Clone Name: OTI4B1
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TPMT (NP_000358) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28.2 kDa
• Gene Name: Homo sapiens thiopurine S-methyltransferase (TPMT), transcript variant 1, mRNA.
• Database Link:
  NP_000358
  Entrez Gene 7172 Human
  UniProt ID P51580
• Background: This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals. A pseudogene for this locus is located on chromosome 18q.
• Synonyms: OTTHUMP00000016076; S-adenosyl-L-methionine:thiopurine S-methyltransferase; thiopurine methyltransferase; thiopurine S-methyltransferase

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - other enzymes