NIT2 Mouse Monoclonal Antibody [Clone ID: OTI6H7]

CAT#: CF501081

Carrier-free (BSA/glycerol-free) NIT2 mouse monoclonal antibody, clone OTI6H7 (formerly 6H7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIT2 (RC210660, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NIT2. Positive lysates LY402761 (100ug) and LC402761 (20ug) can be purchased separately from OriGene.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and NIT2-Knockout 293T cells (KO, Cat# LC812235) were separated by SDS-PAGE and immunoblotted with anti-NIT2 monoclonal antibody TA501081, (1:100). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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  Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-NIT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501081, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-NIT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501081, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-NIT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501081, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-NIT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501081, Dilution 1:50)

• Anti-NIT2 mouse monoclonal antibody (TA501081) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NIT2 (RC210660).

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Specifications

Product Data

• Clone Name: OTI6H7
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NIT2 (NP_064587) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.4 kDa
• Gene Name: Homo sapiens nitrilase family member 2 (NIT2), mRNA.
• Database Link:
  NP_064587
  Entrez Gene 52633 MouseEntrez Gene 288174 RatEntrez Gene 56954 Human
  UniProt ID Q9NQR4
• Background: Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle
• Synonyms: HEL-S-8a