LIPG Mouse Monoclonal Antibody [Clone ID: OTI1E3]

CAT#: CF501082

Carrier-free (BSA/glycerol-free) LIPG mouse monoclonal antibody, clone OTI1E3 (formerly 1E3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIPG (RC209248, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIPG. Positive lysates LY401821 (100ug) and LC401821 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LIPG monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Anti-LIPG mouse monoclonal antibody (TA501082) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIPG (RC209248).

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  HEK293T cells transfected with either RC209248 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIPG antibody (TA501082), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1E3
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:1000, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human LIPG (NP_006024) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.6 kDa
• Gene Name: Homo sapiens lipase G, endothelial type (LIPG), transcript variant 1, mRNA.
• Database Link:
  NP_006024
  Entrez Gene 9388 Human
  UniProt ID Q9Y5X9
• Background: The protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. [provided by RefSeq]
• Synonyms: EDL; EL; PRO719

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Glycerolipid metabolism, Metabolic pathways