NIT1 Mouse Monoclonal Antibody [Clone ID: OTI3A11]

CAT#: CF501084

Carrier-free (BSA/glycerol-free) NIT1 mouse monoclonal antibody, clone OTI3A11 (formerly 3A11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIT1 (RC211519, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NIT1. Positive lysates LY401717 (100ug) and LC401717 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-NIT1 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-NIT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501084, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-NIT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501084, Dilution 1:50)

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  Anti-NIT1 mouse monoclonal antibody (TA501084) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NIT1 (RC211519).

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  Immunofluorescent staining of HT29 cells using anti-NIT1 mouse monoclonal antibody (TA501084).

• HEK293T cells transfected with either pCMV6-ENTRY NIT1 (RC211519) (Red) or empty vector control plasmid (Blue) were immunostained with anti-NIT1 mouse monoclonal (TA501084), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI3A11
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100, Flow 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NIT1 (NP_005591) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.7 kDa
• Gene Name: Homo sapiens nitrilase 1 (NIT1), transcript variant 1, mRNA.
• Database Link:
  NP_005591
  Entrez Gene 27045 MouseEntrez Gene 289222 RatEntrez Gene 4817 Human
  UniProt ID Q86X76
• Background: Play a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. it is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2.
• Synonyms: MGC57670