RhoGDI (ARHGDIA) Mouse Monoclonal Antibody [Clone ID: OTI1F2]

CAT#: CF501089

Carrier-free (BSA/glycerol-free) ARHGDIA mouse monoclonal antibody, clone OTI1F2 (formerly 1F2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARHGDIA (RC200902, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARHGDIA. Positive lysates LY401371 (100ug) and LC401371 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARHGDIA monoclonal antibody.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and ARHGDIA-Knockout 293T cells (KO, Cat# LC811309) were separated by SDS-PAGE and immunoblotted with anti-ARHGDIA monoclonal antibody TA501089, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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  HEK293T cells transfected with either RC200902 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ARHGDIA antibody (TA501089), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-ARHGDIA antibody (TA501089), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-ARHGDIA antibody (TA501089), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI1F2
• Applications: FC, WB
• Recommended Dilution: WB 1:200~1000, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ARHGDIA (NP_004300) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23.0 kDa
• Gene Name: Homo sapiens Rho GDP dissociation inhibitor alpha (ARHGDIA), transcript variant 2, mRNA.
• Database Link:
  NP_004300
  Entrez Gene 192662 MouseEntrez Gene 360678 RatEntrez Gene 475924 DogEntrez Gene 714752 MonkeyEntrez Gene 396 Human
  UniProt ID P52565
• Background: Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA (Leffers et al., 1993 [PubMed 8262133]). [supplied by OMIM]
• Synonyms: GDIA1; HEL-S-47e; NPHS8; RHOGDI; RHOGDI-1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Neurotrophin signaling pathway