TUBA8 Mouse Monoclonal Antibody [Clone ID: OTI2G6]

CAT#: CF501096

Carrier-free (BSA/glycerol-free) TUBA8 mouse monoclonal antibody, clone OTI2G6 (formerly 2G6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBA8 (RC211175, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBA8. Positive lysates LY412867 (100ug) and LC412867 (20ug) can be purchased separately from OriGene.

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  WB analysis of extracts (35ug) from 9 different cell lines by using anti-TUBA8 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-TUBA8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501096, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-TUBA8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501096, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-TUBA8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501096, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-TUBA8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501096, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TUBA8 mouse monoclonal antibody. (TA501096)

• Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-TUBA8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501096, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-TUBA8 mouse monoclonal antibody. (TA501096)

• Anti-TUBA8 mouse monoclonal antibody (TA501096) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TUBA8 (RC211175).

• Immunoprecipitation (IP) of TUBA8 by using TrueMab monoclonal anti-TUBA8 antibodies (Negative control: IP without adding anti-TUBA8 antibody.). For each experiment, 500ul of DDK tagged TUBA8 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-TUBA8 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

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Specifications

Product Data

• Clone Name: OTI2G6
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB 1:2000, IHC: 1:50-1:150, IF 1:100, Flow 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TUBA8(NP_061816) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50.1 kDa
• Gene Name: Homo sapiens tubulin alpha 8 (TUBA8), transcript variant 1, mRNA.
• Database Link:
  NP_061816
  Entrez Gene 500377 RatEntrez Gene 486762 DogEntrez Gene 710378 MonkeyEntrez Gene 51807 Human
  UniProt ID Q9NY65
• Background: Microtubules are cylindrical tubes of 20-25 nm in diameter. They are composed of protofilaments which are in turn composed of alpha- and beta-tubulin polymers. Each microtubule is polarized, at one end alpha-subunits are exposed (-) and at the other beta-subunits are exposed (+). Microtubules act as a scaffold to determine cell shape, and provide a backbone for cell organelles and vesicles to move on, a process that requires motor proteins. The major microtubule motor proteins are kinesin, which generally moves towards the (+) end of the microtubule, and dynein, which generally moves towards the (-) end. Microtubules also form the spindle fibers for separating chromosomes during mitosis.
• Synonyms: TUBAL2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection